TrkB Rekombinanter Antikörper

TrkB Rekombinant Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241473G5

Kat-Nr. : 80878-6-PBS

Synonyme

NTRK2, 241473G5, BDNF/NT-3 growth factors receptor, GP145 TrkB, GP145-TrkB

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  WB analysis using 80878-6-RR (same clone as 80878-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80878-6-RR (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IP experiment of mouse cerebellum using 80878-6-RR (same clone as 80878-6-PBS)

  IP result of anti-TrkB (IP:80878-6-RR, 4ug; Detection:80878-6-RR 1:4000) with mouse cerebellum tissue lysate 2000 ug. This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80878-6-RR (same clone as 80878-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80878-6-RR (same clone as 80878-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 80878-6-RR (same clone as 80878-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TrkB antibody (80878-6-RR, Clone: 241473G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80878-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80878-6-RR against Human TrkB were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

80878-6-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA TrkB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TrkB fusion protein Ag3790
• Vollständiger Name: neurotrophic tyrosine kinase, receptor, type 2
• Berechnetes Molekulargewicht: 822 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: BC031835
• Gene symbol: TrkB
• Gene ID (NCBI): 4915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).