TrkB Rekombinanter Antikörper

TrkB Rekombinant Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

241473G5

Rezensionen (1)

Kat-Nr. : 80878-6-RR

Synonyme

NTRK2, 241473G5, BDNF/NT-3 growth factors receptor, GP145 TrkB, GP145-TrkB

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  WB analysis using 80878-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80878-6-RR (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 80878-6-RR

  IP result of anti-TrkB (IP:80878-6-RR, 4ug; Detection:80878-6-RR 1:4000) with mouse cerebellum tissue lysate 2000 ug.

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  IHC staining of rat brain using 80878-6-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 80878-6-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 80878-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TrkB antibody (80878-6-RR, Clone: 241473G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 80878-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80878-6-RR against Human TrkB were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maus-Cerebellum-Gewebe
• Erfolgreiche IP: Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80878-6-RR bindet in WB, IHC, IF-P, IP, ELISA TrkB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TrkB fusion protein Ag3790
• Vollständiger Name: neurotrophic tyrosine kinase, receptor, type 2
• Berechnetes Molekulargewicht: 822 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: BC031835
• Gene symbol: TrkB
• Gene ID (NCBI): 4915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-24-2025)

TRKB (in green) worked perfectly in the human TSC FFPE cortical tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

TrkB Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:80878-6-RR)