TrpRS Monoklonaler Antikörper

TrpRS Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6C3

Kat-Nr. : 67952-1-PBS

Synonyme

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

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  WB analysis using 67952-1-Ig (same clone as 67952-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67952-1-Ig (same clone as 67952-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67952-1-Ig (same clone as 67952-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67952-1-Ig (same clone as 67952-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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  IF Staining of LNCaP using 67952-1-Ig (same clone as 67952-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67952-1-PBS bindet in WB, IF/ICC, Indirect ELISA TrpRS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TrpRS fusion protein Ag31430
• Vollständiger Name: tryptophanyl-tRNA synthetase
• Berechnetes Molekulargewicht: 471 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC017489
• Gene symbol: WARS1
• Gene ID (NCBI): 7453
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).