Tuberin/TSC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Tuberin/TSC2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24601-1-PBS

Synonyme

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  WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

  WB result of Tuberin/TSC2 antibody (24601-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 24601-1-AP (same clone as 24601-1-PBS)

  IP result of anti-Tuberin/TSC2 (IP:24601-1-AP, 4ug; Detection:24601-1-AP 1:1000) with SH-SY5Y cells lysate 1080 ug. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 24601-1-AP (same clone as 24601-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Tuberin/TSC2 antibody (24601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Tuberin (24601-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24601-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Tuberin/TSC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Tuberin/TSC2 fusion protein Ag18133
• Vollständiger Name: tuberous sclerosis 2
• Berechnetes Molekulargewicht: 1807 aa, 201 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC150300
• Gene symbol: TSC2
• Gene ID (NCBI): 7249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors.