Alpha Tubulin Monoklonaler Antikörper

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• KD/KO Validated

Alpha Tubulin Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, CoIP

Konjugation

HRP

Publikationen(154)

CloneNo.

1E4C11

Rezensionen (1)

Kat-Nr. : HRP-66031

Synonyme

TUBA1B, Tubulin alpha 1B chain, 1E4C11, Alpha-tubulin ubiquitous, EC:3.6.5.-

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  WB analysis of multi-cells/tissue using HRP-66031

  Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody HRP-60031 at a dilution of 1:5000.

• 

  WB analysis of HeLa using HRP-66031

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-66031 (Alpha Tubulin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 143 publications below
• CoIP: See 1 publications below

Produktinformation

HRP-66031 bindet in WB, CoIP Alpha Tubulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: tubulin, alpha 1b
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC009314
• Gene symbol: Alpha Tubulin
• Gene ID (NCBI): 10376
• Konjugation: HRP
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the function of alpha-tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport, and are also involved in cell division.

Where is alpha-tubulin expressed?

Alpha-tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.

What are the post-translational modifications of alpha-tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerized microtubule. For example, the first modification to be identified was detyrosination,¹ as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyrosination along the structure, regulating protein interactions and allowing subcellular compartments to be defined.^2,3 Polyglutamylation also occurs at several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

1. Gundersen, G. G., Khawaja, S. &  Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a  mechanism for subcellular differentiation of microtubules. J. Cell  Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins  and Their Interactions at Microtubule Ends. Curr. Biol. 20,  R528-R537 (2010).

3. Jiang, K. & Akhmanova, A.  Microtubule tip-interacting proteins: a view from both ends. Curr.  Opin. Cell Biol. 23, 94-101 (2011).

Publikationen

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	WB	Cell; Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.; Authors - Shicheng Su
rat	WB	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
human	WB	Immunity; Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals.; Authors - Yang Qiu
mouse	WB	Immunity; Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis.; Authors - Xuguang Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (10-05-2024)

I love Proteintech HRP-conjugated antibodies. These are def my favourites for WB controls (housekeeping proteins), I have got actin, tubulin and GAPDH. Very good bands, super reliable.

• Applications: Western Blot
• Cell Tissue Type: Mouse