Tubulin Beta Class V Monoklonaler Antikörper

Tubulin Beta Class V Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D7B9

Kat-Nr. : 66362-1-PBS

Synonyme

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

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  WB analysis of HeLa using 66362-1-Ig (same clone as 66362-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66362-1-Ig (same clone as 66362-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66362-1-Ig (same clone as 66362-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66362-1-Ig (same clone as 66362-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66362-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Tubulin Beta Class V und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin, beta 6
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: NM_032525
• Gene symbol: Beta Tubulin
• Gene ID (NCBI): 84617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.