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Tyrosinase Rekombinanter Antikörper

Tyrosinase Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242174D2

Kat-Nr. : 84723-3-PBS

Synonyme

TYR, 242174D2, EC:1.14.18.1, LB24 AB, LB24-AB

Filter:
  • Western Blot (WB) analysis of various lysates using Tyrosinase Recombinant antibody (84723-3-RR)

    WB analysis using 84723-3-RR (same clone as 84723-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84723-3-RR (Tyrosinase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84723-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84723-3-RR

    Affinity and Kinetic Characterization of 84723-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84723-3-RR against Human Tyrosinase were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84723-3-PBS bindet in WB, Indirect ELISA Tyrosinase und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Tyrosinase fusion protein Ag34940
Vollständiger Name tyrosinase (oculocutaneous albinism IA)
Berechnetes Molekulargewicht 529 aa, 60 kDa
Beobachtetes Molekulargewicht 80 kDa
GenBank-ZugangsnummerBC027179
Gene symbol TYR
Gene ID (NCBI) 7299
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tyrosinase is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. It's a melanocyte-specific enzyme critical for the synthesis of melanin, a process normally restricted to a post-Golgi compartment termed the melanosome. Loss-of-function mutations in tyrosinase are the cause of oculocutaneous albinism. The 60-kDa tyrosinase core polypeptide is modified in the endoplasmic reticulum (ER) by cotranslational addition of multiple N-linked glycans, producing the 70-kDa species. Complex sugar modifications in the Golgi apparatus further increases tyrosinase's molecular mass to 80 kDa, the size of the mature wild-type (WT) isoform. (PMID:10823941)