U2AF2/U2AF65 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

U2AF2/U2AF65 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, RIP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 15624-1-AP

Synonyme

hU2AF(65), hU2AF65, U2AF2, U2AF65

---

• 

  WB analysis of HepG2 using 15624-1-AP

  WB result of U2AF65 antibody (15624-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF65 transfected HepG2 cells.

• 

  WB analysis of Jurkat using 15624-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15624-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15624-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 15624-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 15624-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 15624-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15624-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Produktinformation

15624-1-AP bindet in WB, IF, RIP, IHC, ELISA U2AF2/U2AF65 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: U2AF2/U2AF65 fusion protein Ag8070
• Vollständiger Name: U2 small nuclear RNA auxiliary factor 2
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC008740
• Gene symbol: U2AF2
• Gene ID (NCBI): 11338
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Publikationen

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
mouse	IF,RIP	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
human	WB	Sci Rep; Identification of novel PANDAR protein interaction partners involved in splicing regulation.; Authors - N Pospiech
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	J Immunol Res; Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.; Authors - Li Fang
human	WB	Cell Death Dis; RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.; Authors - Shicheng Sun