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U2AF2/U2AF65 Monoklonaler Antikörper

U2AF2/U2AF65 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

1B7E7

Kat-Nr. : CL488-68166

Synonyme

U2AF65, U2AF2, hU2AF65, hU2AF(65), 1B7E7

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated U2AF2/U2AF65 Monoclo (CL488-68166)

    IF Staining of HEK-293 using CL488-68166

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 U2AF2 antibody (CL488-68166, Clone: 1B7E7 ) at dilution of 1:100, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated U2AF2/U2AF65 Monoclo (CL488-68166)

    FC experiment of HEK-293 using CL488-68166

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human U2AF2 (CL488-68166, Clone:1B7E7) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHEK-293-Zellen
Erfolgreiche Detektion in FC (Intra)HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68166 bindet in IF/ICC, FC (Intra) U2AF2/U2AF65 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen U2AF2/U2AF65 fusion protein Ag8070
Vollständiger Name U2 small nuclear RNA auxiliary factor 2
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht60-65 kDa
GenBank-ZugangsnummerBC008740
Gene symbol U2AF2
Gene ID (NCBI) 11338
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 U2AF2/U2AF65 antibody CL488-68166Protokoll herunterladen
FC protocol for CL Plus 488 U2AF2/U2AF65 antibody CL488-68166Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human

Diabet Med

LncRNA CASC2 mediates SOCS6 mRNA stabilization via U2AF2 recruitment to modulate macrophage polarisation in diabetic retinopathy

Authors - Fan Xiao
View Article