U2AF35 Polyklonaler Antikörper

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• KD/KO Validated

U2AF35 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10334-1-AP

Synonyme

FP793, RN, RNU2AF1, U2AF1, U2AF35, U2AFBP

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10334-1-AP

  WB result of U2AF35 antibody (10334-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF35 transfected HeLa cells.

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  WB analysis of COLO 320 using 10334-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10334-1-AP (U2AF35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10334-1-AP (U2AF35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10334-1-AP (U2AF35 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 10334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10334-1-AP (U2AF35 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below

Produktinformation

10334-1-AP bindet in WB, IHC, ELISA U2AF35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: U2AF35 fusion protein Ag0399
• Vollständiger Name: U2 small nuclear RNA auxiliary factor 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC001923
• Gene symbol: U2AF1
• Gene ID (NCBI): 7307
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.

Publikationen

Species	Application	Title
human	WB	Mol Cell; U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.; Authors - Sung Mi Park
human	WB	Nucleic Acids Res; PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.; Authors - Jana Královicová
human	WB	Cancer Res; PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.; Authors - Yi-Zi Zheng
human		Nucleic Acids Res; Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins.; Authors - Jana Kralovicova
human	WB	Int J Mol Sci; Splicing Enhancers at Intron-Exon Borders Participate in Acceptor Splice Sites Recognition.; Authors - Tatiana Kováčová
human	WB	J Biol Chem; Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3.; Authors - Little John T JT