UACA Polyklonaler Antikörper

UACA Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25654-1-AP

Synonyme

KIAA1561, NUCLING, Uveal autoantigen with coiled-coil domains and ankyrin repeats

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  WB analysis using 25654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 25654-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25654-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25654-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A375 using 25654-1-AP

  Immunofluorescent analysis of A375 cells using 25654-1-AP (UACA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, HeLa-Zellen, Neuro-2a-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

25654-1-AP bindet in WB, IHC, IF/ICC, ELISA UACA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UACA fusion protein Ag22438
• Vollständiger Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
• Berechnetes Molekulargewicht: 1416 aa, 163 kDa
• Beobachtetes Molekulargewicht: 163 kDa
• GenBank-Zugangsnummer: BC113407
• Gene symbol: UACA
• Gene ID (NCBI): 55075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells; Authors - Hewei Zhang
mouse	IF	J Zhejiang Univ Sci B; Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification; Authors - Tiantian Ge
human	WB	J Oncol; Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling.; Authors - Yongqiang Wang
canine	WB	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui
human	WB,IF	Int J Clin Exp Pathol; Knockdown of UACA inhibitsproliferation and invasion and promotes senescence of hepatocellular carcinoma cells; Authors - Jing-Yuan Sun ; KD Validated