UAP1L1 Polyklonaler Antikörper

UAP1L1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25262-1-AP

Synonyme

UDP-N-acetylhexosamine pyrophosphorylase-like protein 1, EC:2.7.7.-

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  WB analysis of MDA-MB-453s using 25262-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 25262-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25262-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25262-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 25262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 25262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25262-1-AP

  Immunofluorescent analysis of HepG2 cells using 25262-1-AP (UAP1L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, A375-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25262-1-AP bindet in WB, IHC, IF/ICC, ELISA UAP1L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: UDP-N-acteylglucosamine pyrophosphorylase 1-like 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-59 kDa
• GenBank-Zugangsnummer: NM_207309
• Gene symbol: UAP1L1
• Gene ID (NCBI): 91373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Heliyon; Changes in transcriptomic landscape with macronutrients intake switch are independent from O-GlcNAcylation levels in heart throughout postnatal development in rats; Authors - Antoine Persello