UBA3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBA3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13857-1-AP

Synonyme

EC:6.2.1.64, hUBA3, NEDD8 activating enzyme E1C, NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C

---

• 

  WB analysis using 13857-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13857-1-AP (UBA3 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13857-1-AP

  WB result of UBA3 antibody (13857-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA3 transfected HEK-293 cells.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13857-1-AP bindet in WB, ELISA UBA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBA3 fusion protein Ag4831
• Vollständiger Name: ubiquitin-like modifier activating enzyme 3
• Berechnetes Molekulargewicht: 463 aa, 52 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC022853
• Gene symbol: UBA3
• Gene ID (NCBI): 9039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.