UBA6 Polyklonaler Antikörper

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• KD/KO Validated

UBA6 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13211-1-AP

Synonyme

E1 L2, Monocyte protein 4, MOP 4, MOP4, UBA6, UBE1L2, Ubiquitin activating enzyme 6

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Galerie der Validierungsdaten

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  WB analysis using 13211-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13211-1-AP

  WB result of UBA6 antibody (13211-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA6 transfected HEK-293 cells.

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  WB analysis of human brain using 13211-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 13211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 13211-1-AP (UBA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

13211-1-AP bindet in WB, IP, IHC, IF, ELISA UBA6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBA6 fusion protein Ag4000
• Vollständiger Name: ubiquitin-like modifier activating enzyme 6
• Berechnetes Molekulargewicht: 1052 aa, 118 kDa
• Beobachtetes Molekulargewicht: 118 kDa
• GenBank-Zugangsnummer: BC031637
• Gene symbol: UBA6
• Gene ID (NCBI): 55236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBA6(Ubiquitin-like modifier-activating enzyme 6) is also named as MOP4, UBE1L2 and belongs to the ubiquitin-activating E1 family. It probably acts as a single molecule like UBE1 and not as a heterodimeric E1 complex, as formed by AOS1/Uba2 and APP-BP1/Uba3. UBA6 is even more restricted in distribution because it is only found in vertebrates but not in lower organisms so that it might have a specialized role in tissues of higher organisms, and in particular, in the testis, where it is expressed by far most abundantly.(PMID:17580310). It has 4 isoforms produced by alternative splicing. This antibody recognizes the full-length(118 kda) and the truncated isoform CRA_b (60-70 kDa) of UBA6 (NCBI).

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IP	Nat Commun; Inhibition of UBA6 by inosine augments tumour immunogenicity and responses; Authors - Lei Zhang