UBAC2 Polyklonaler Antikörper

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• KD/KO Validated

UBAC2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 25122-1-AP

Synonyme

PHGDHL1, Phosphoglycerate dehydrogenase-like protein 1, PSEC0110, UBA domain-containing protein 2, UBAC 2

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  WB analysis of MCF-7 using 25122-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25122-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 25122-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UBAC2 antibody (25122-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:3000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Produktinformation

25122-1-AP bindet in WB, IHC, IF/ICC, RIP, ELISA UBAC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBAC2 fusion protein Ag19142
• Vollständiger Name: UBA domain containing 2
• Berechnetes Molekulargewicht: 344 aa, 39 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC121139
• Gene symbol: UBAC2
• Gene ID (NCBI): 337867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
human	WB,RIP,IHC,IF	Cell Death Dis; UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis.; Authors - Chaohui Gu ; KD Validated
human	WB	Mol Cell; TMUB1 is an endoplasmic reticulum-resident escortase that promotes the p97-mediated extraction of membrane proteins for degradation; Authors - Linhan Wang
human	WB,IF	EMBO J; ER-phagy restrains inflammatory responses through its receptor UBAC2; Authors - Xing He