UBAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBAP1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 12385-1-AP

Synonyme

NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1

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Galerie der Validierungsdaten

• 

  WB analysis of human heart using 12385-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12385-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12385-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12385-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12385-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12385-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12385-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12385-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12385-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12385-1-AP

  IP Result of anti-UBAP1 (IP:12385-1-AP, 4ug; Detection:12385-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12385-1-AP

  Immunofluorescent analysis of Hela cells, using UBAP1 antibody 12385-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, A431-Zellen, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, Maushirngewebe, Maushodengewebe, Mauslungengewebe, Maus-Thymusgewebe, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Herzgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IP: See 2 publications below

Produktinformation

12385-1-AP bindet in WB, IP, IHC, IF UBAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBAP1 fusion protein Ag3055
• Vollständiger Name: ubiquitin associated protein 1
• Berechnetes Molekulargewicht: 502 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC020950
• Gene symbol: UBAP1
• Gene ID (NCBI): 51271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

Publikationen

Species	Application	Title
human	WB	EMBO J; A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.; Authors - Paul Rc Imbert
human	WB	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
human	WB	J Cell Biol; VPS37A directs ESCRT recruitment for phagophore closure.; Authors - Yoshinori Takahashi
human	WB	Cell Rep; The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.; Authors - Daniel S J Miller
human	WB, IP	Cell Rep; Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.; Authors - Dmitri Kharitidi
human	WB,IP	PLoS Pathog; A cytoplasmic tail determinant in HIV-1 Vpu mediates targeting of tetherin for endosomal degradation and counteracts interferon-induced restriction.; Authors - Kueck Tonya T

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