UBAP2 Rekombinanter Antikörper

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UBAP2 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240890F6

Kat-Nr. : 83885-2-PBS

Synonyme

240890F6, bA176F3.5, KIAA1491, UBAP 2, ubiquitin associated protein 2

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  WB analysis of HeLa using 83885-2-RR (same clone as 83885-2-PBS)

  WB result of UBAP2 antibody (83885-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBAP2 transfected HeLa cells. This data was developed using the same antibody clone with 83885-2-PBS in a different storage buffer formulation.

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  WB analysis using 83885-2-RR (same clone as 83885-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83885-2-RR (UBAP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83885-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83885-2-RR (same clone as 83885-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBAP2 antibody (83885-2-RR, Clone: 240890F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83885-2-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 83885-2-RR (same clone as 83885-2-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug UBAP2 Recombinant antibody (83885-2-RR, Clone:240890F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83885-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00850-2

  Cytometric bead array standard curve of MP00850-2, UBAP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83885-2-PBS. Detection antibody: 83885-1-PBS. Standard: Ag30572. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00850-3

  Cytometric bead array standard curve of MP00850-3, UBAP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83885-2-PBS. Detection antibody: 83885-4-PBS. Standard: Ag30572. Range: 1.25-80 ng/mL.

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  Affinity and Kinetic Characterization of 83885-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83885-2-RR against Human UBAP2 were performed. The affinity constant is 0.59 nM.

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Geprüfte Anwendungen

Produktinformation

83885-2-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA UBAP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UBAP2 fusion protein Ag30572
• Vollständiger Name: ubiquitin associated protein 2
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_018449
• Gene symbol: UBAP2
• Gene ID (NCBI): 55833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.