UBAP2 Rekombinanter Antikörper

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• KD/KO Validated

UBAP2 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240890F6

Kat-Nr. : 83885-2-RR

Synonyme

ubiquitin associated protein 2, UBAP 2, KIAA1491, bA176F3.5, 240890F6

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  WB analysis of HeLa using 83885-2-RR

  WB result of UBAP2 antibody (83885-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBAP2 transfected HeLa cells.

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  WB analysis using 83885-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83885-2-RR (UBAP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83885-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBAP2 antibody (83885-2-RR, Clone: 240890F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U-2 OS using 83885-2-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug UBAP2 Recombinant antibody (83885-2-RR, Clone:240890F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83885-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83885-2-RR against Human UBAP2 were performed. The affinity constant is 0.59 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HCT 116-Zellen, HEK-293T-Zellen, Jurkat-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): U-2 OS

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83885-2-RR bindet in WB, IF/ICC, FC (Intra), ELISA UBAP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UBAP2 fusion protein Ag30572
• Vollständiger Name: ubiquitin associated protein 2
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_018449
• Gene symbol: UBAP2
• Gene ID (NCBI): 55833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.