UBASH3A-Specific Polyklonaler Antikörper

UBASH3A-Specific Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 15823-1-AP

Synonyme

Cbl interacting protein 4, CLIP4, STS 2, STS2, T cell ubiquitin ligand, TULA, UBASH3A, UBASH3A-Specific



Geprüfte Anwendungen

Erfolgreiche Detektion in WBmouse bone marrow
Erfolgreiche IPMausmilzgewebe
Erfolgreiche Detektion in IF/ICCTHP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 7 publications below

Produktinformation

15823-1-AP bindet in WB, IF/ICC, IP, ELISA UBASH3A-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name ubiquitin associated and SH3 domain containing, A
Berechnetes Molekulargewicht 74 kDa
Beobachtetes Molekulargewicht 70-74 kDa
GenBank-ZugangsnummerNM_018961
Gene symbol UBASH3A
Gene ID (NCBI) 53347
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for UBASH3A-Specific antibody 15823-1-APProtokoll herunterladen
IF protocol for UBASH3A-Specific antibody 15823-1-APProtokoll herunterladen
IP protocol for UBASH3A-Specific antibody 15823-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Diabetes

UBASH3A Mediates Risk for Type 1 Diabetes Through Inhibition of T-Cell Receptor-Induced NF-κB Signaling.

Authors - Yan Ge
humanWB

Int J Mol Sci

UBASH3A Interacts with PTPN22 to Regulate IL2 Expression and Risk for Type 1 Diabetes

Authors - Jeremy R B Newman
humanWB

BMC Cancer

FLI1 induces erythroleukemia through opposing effects on UBASH3A and UBASH3B expression

Authors - Jie Wang
humanWB

Immunity

TCR signaling promotes formation of an STS1-Cbl-b complex with pH-sensitive phosphatase activity that suppresses T cell function in acidic environments

Authors - Yuan-Li Tsai
humanWB

J Immunol

UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes

Authors - Yan Ge
mouseWB

Sci Signal

Inhibition of T cell activation and function by the adaptor protein CIN85.

Authors - Mei Suen Kong