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UBASH3A-Specific Polyklonaler Antikörper

UBASH3A-Specific Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15823-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using UBASH3A-Specific Polyclonal antibody (15823-1-AP)

    WB analysis using 15823-1-AP (same clone as 15823-1-PBS)

    mouse bone marrow tissue were subjected to SDS PAGE followed by western blot with 15823-1-AP (UBASH3A-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using UBASH3A-Specific Polyclonal antibody (15823-1-AP)

    IP experiment of mouse spleen using 15823-1-AP (same clone as 15823-1-PBS)

    IP result of anti-UBASH3A-Specific (IP:15823-1-AP, 4ug; Detection:15823-1-AP 1:500) with mouse spleen tissue lysate 1920 ug. This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using UBASH3A-Specific Polyclonal antibody (15823-1-AP)

    IF Staining of THP-1 using 15823-1-AP (same clone as 15823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using UBASH3A-Specific antibody (15823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15823-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA UBASH3A-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name ubiquitin associated and SH3 domain containing, A
Berechnetes Molekulargewicht 74 kDa
Beobachtetes Molekulargewicht 70-74 kDa
GenBank-ZugangsnummerNM_018961
Gene symbol UBASH3A
Gene ID (NCBI) 53347
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific.