UBC12 Monoklonaler Antikörper

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UBC12 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H8D2

Kat-Nr. : 67482-1-PBS

Synonyme

UBE2M, EC:2.3.2.34, hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12

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  WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67482-1-Ig (UBC12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

  WB result of UBC12 antibody (67482-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC12 antibody (67482-1-Ig, Clone: 1H8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67482-1-PBS bindet in WB, IHC, IF/ICC, ELISA UBC12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UBC12 fusion protein Ag5984
• Vollständiger Name: ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC058924
• Gene symbol: UBC12
• Gene ID (NCBI): 9040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.