UBC12 Rekombinanter Antikörper

UBC12 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240477H5

Kat-Nr. : 83628-1-RR

Synonyme

UBE2M, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein, hUbc12

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  WB analysis using 83628-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83628-1-RR (UBC12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 83628-1-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug UBC12 Recombinant antibody (83628-1-RR, Clone:240477H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83628-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83628-1-RR against Human UBC12 were performed. The affinity constant is 2.45 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HT-1080.Zellen, L02-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83628-1-RR bindet in WB, FC (Intra), ELISA UBC12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UBC12 fusion protein Ag5984
• Vollständiger Name: ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC058924
• Gene symbol: UBC12
• Gene ID (NCBI): 9040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2M (Ubiquitin-conjugating enzyme E2 M), also called UBC12, accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction between UBE2M and the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (PMID: 10207026; 15361859).