UBC9 Polyklonaler Antikörper

UBC9 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10070-1-AP

Synonyme

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

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Galerie der Validierungsdaten

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  WB analysis using 10070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10070-1-AP (UBC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10070-1-AP (UBC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10070-1-AP (UBC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10070-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10070-1-AP (UBC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC9 antibody (10070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

10070-1-AP bindet in WB, IP, IHC, IF, ELISA UBC9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBC9 fusion protein Ag0111
• Vollständiger Name: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC051289
• Gene symbol: UBE2I
• Gene ID (NCBI): 7329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C.; Authors - Zhong Nanbert N
human	WB,IP	Biochem Biophys Res Commun; SUMOylation of optineurin is critical for inhibiting interferon β production; Authors - Xifeng Hu
	WB	J Virol; Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication; Authors - Xiangju Wu