UBC9 Polyklonaler Antikörper

UBC9 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10224-1-AP

Synonyme

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

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Galerie der Validierungsdaten

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  WB analysis of mouse spleen using 10224-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10224-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10224-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10224-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10224-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10224-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10224-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10224-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, HEK-293-Zellen, humanes Hodengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10224-1-AP bindet in WB, IHC, ELISA UBC9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBC9 fusion protein Ag0252
• Vollständiger Name: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000427
• Gene symbol: UBE2I
• Gene ID (NCBI): 7329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).