UBC9 Monoklonaler Antikörper

UBC9 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A10B11

Kat-Nr. : 60201-1-PBS

Synonyme

UBE2I, 4A10B11, C358B7.1, E2,UBE2I, EC:2.3.2.-

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  WB analysis using 60201-1-Ig (same clone as 60201-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 60201-1-Ig (same clone as 60201-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60201-1-PBS bindet in WB, IHC, Indirect ELISA UBC9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UBC9 fusion protein Ag7885
• Vollständiger Name: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC004437
• Gene symbol: UBC9
• Gene ID (NCBI): 7329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).