FAT10 Polyklonaler Antikörper

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• KD/KO Validated

FAT10 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 13003-2-AP

Synonyme

Diubiquitin, FAT10, GABBR1, UBD, UBD 3, ubiquitin D, Ubiquitin like protein FAT10

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  WB analysis using 13003-2-AP

  TNF alpha and IFN gamma treated HepG2 cells were subjected to SDS PAGE followed by western blot with 13003-2-AP (FAT10 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 13003-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13003-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13003-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13003-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: TNF alpha and IFN gamma treated HepG2 cells
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, humanes Lymphomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

13003-2-AP bindet in WB, IHC, IF, CoIP, ELISA FAT10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAT10 fusion protein Ag3680
• Vollständiger Name: ubiquitin D
• Berechnetes Molekulargewicht: 165 aa, 18 kDa
• Beobachtetes Molekulargewicht: 20 kDa, 70kDa
• GenBank-Zugangsnummer: BC012472
• Gene symbol: FAT10
• Gene ID (NCBI): 10537
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAT10, also named UBD, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier that can be covalently attached to the target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. FAT10 also has important roles in cell mitosis, chromosome instability, apoptosis, and immune response. FAT10 mediates apoptosis in a caspase-dependent manner, especially in the renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). FAT10 regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. FAT10 may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full-length FAT10 of human origin.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Cancer Res; The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.; Authors - Xiuxia Liu ; KD Validated
mouse	WB,IF	J Biol Chem; Ubiquitin D regulates IRE1α/JNK-dependent apoptosis in pancreatic beta cells.; Authors - Flora Brozzi
human	WB	Environ Toxicol; Increased UBD Is a Potential Diagnostic and Prognostic Biomarker in Glioma; Authors - Tao Wu
mouse	IHC	DNA Res; Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.; Authors - Kobayashi Atsushi A
rat	IHC	Toxicol Lett; Ochratoxin A induces karyomegaly and cell cycle aberrations in renal tubular cells without relation to induction of oxidative stress responses in rats.; Authors - Taniai Eriko E
human	IHC	Discov Oncol; Transcription factor networks and novel immune biomarkers reveal key prognostic and therapeutic insights in ovarian cancer; Authors - Aiqin Zhao