UBE2C Polyklonaler Antikörper

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• KD/KO Validated

UBE2C Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 12134-2-AP

Synonyme

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12134-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12134-2-AP (UBE2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12134-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12134-2-AP (UBE2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12134-2-AP

  IP Result of anti-UBE2C (IP:12134-2-AP, 3ug; Detection:12134-2-AP 1:300) with HeLa cells lysate 2440ug.

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  IHC staining of human colon cancer using 12134-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12134-2-AP (UBE2C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12134-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12134-2-AP (UBE2C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

12134-2-AP bindet in WB, IP, IHC, IF, ELISA UBE2C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2C fusion protein Ag2780
• Vollständiger Name: ubiquitin-conjugating enzyme E2C
• Berechnetes Molekulargewicht: 179 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC016292
• Gene symbol: UBE2C
• Gene ID (NCBI): 11065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation (PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

Publikationen

Species	Application	Title
	WB	Plant Cell; SINAT E3 Ubiquitin Ligases Mediate FREE1 and VPS23A Degradation to Modulate Abscisic Acid Signaling.; Authors - Fan-Nv Xia
human	WB,IF	Arterioscler Thromb Vasc Biol; Disturbed Flow Increases UBE2C (Ubiquitin E2 Ligase C) via Loss of miR-483-3p, Inducing Aortic Valve Calcification by the pVHL (von Hippel-Lindau Protein) and HIF-1α (Hypoxia-Inducible Factor-1α) Pathway in Endothelial Cells.; Authors - Joan Fernandez Esmerats
human	WB,IHC	Cell Biosci; DNA primase subunit 1 deteriorated progression of hepatocellular carcinoma by activating AKT/mTOR signaling and UBE2C-mediated P53 ubiquitination.; Authors - Mengqi Zhu
human	WB	Biomed Pharmacother; Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.; Authors - Shuai Huang
mouse	WB，IHC	Front Genet; Identifying the Key Genes in Mouse Liver Regeneration After Partial Hepatectomy by Bioinformatics Analysis and in vitro/vivo Experiments.; Authors - Jian Zhao
human	WB,IHC	Am J Transl Res; RIZ1 negatively regulates ubiquitin-conjugating enzyme E2C/UbcH10 via targeting c-Myc in meningioma.; Authors - Zheng Cai ; KD Validated