UBE2D1/2/3/4 Polyklonaler Antikörper

UBE2D1/2/3/4 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11373-1-PBS

Synonyme

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  WB analysis using 11373-1-AP (same clone as 11373-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 11373-1-AP (same clone as 11373-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:500. . This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11373-1-AP (same clone as 11373-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 11373-1-AP (same clone as 11373-1-PBS)

  IP result of anti-UBE2D1/2/3/4 (IP:11373-1-AP, 4ug; Detection:11373-1-AP 1:300) with Jurkat cells lysate 2600 ug. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11373-1-AP (same clone as 11373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11373-1-PBS bindet in WB, IHC, IP, Indirect ELISA UBE2D1/2/3/4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2D1/2/3/4 fusion protein Ag1932
• Vollständiger Name: ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC015997
• Gene symbol: UBE2D1
• Gene ID (NCBI): 7321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.