UBE2F Polyklonaler Antikörper

UBE2F Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17056-1-PBS

Synonyme

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  WB analysis using 17056-1-AP (same clone as 17056-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 17056-1-AP (same clone as 17056-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 17056-1-AP (same clone as 17056-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 17056-1-AP (same clone as 17056-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 17056-1-AP (UBE2F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17056-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA UBE2F und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2F fusion protein Ag10764
• Vollständiger Name: ubiquitin-conjugating enzyme E2F (putative)
• Berechnetes Molekulargewicht: 185 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC010549
• Gene symbol: UBE2F
• Gene ID (NCBI): 140739
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).