UBC9-Specific Polyklonaler Antikörper

UBC9-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14837-1-AP

Synonyme

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBC9-Specific, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, Jurkat-Zellen, U-937-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14837-1-AP bindet in WB, IP, IF, IHC, ELISA UBC9-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Berechnetes Molekulargewicht 18 kDa
Beobachtetes Molekulargewicht 18 kDa
GenBank-ZugangsnummerNM_003345
Gene symbol UBC9
Gene ID (NCBI) 7329
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for UBC9-Specific antibody 14837-1-APProtokoll herunterladen
IHC protocol for UBC9-Specific antibody 14837-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
mouse,humanWB,IP

Cell Rep

SUMOylation controls Hu antigen R posttranscriptional activity in liver cancer

Authors - Sofia Lachiondo-Ortega
humanWB

J Ethnopharmacol

miR-122-3p targets UBE2I to regulate the immunosuppression of liver cancer and the intervention of Liujunzi formula

Authors - Zhenhui Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Praveen (Verified Customer) (03-13-2019)

Excellent

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
FH

Shubham (Verified Customer) (03-12-2019)

Very good antibody with a prominent band.

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
UBC9-Specific Antibody Western Blot, validation ( dilution) in AC16 (Cat no:14837-1-AP)