UBE2L3 Polyklonaler Antikörper

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UBE2L3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14415-1-PBS

Synonyme

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  WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 14415-1-AP (same clone as 14415-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 14415-1-AP (same clone as 14415-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14415-1-AP (same clone as 14415-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 14415-1-AP (same clone as 14415-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 14415-1-AP (same clone as 14415-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 14415-1-AP (same clone as 14415-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14415-1-AP (same clone as 14415-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

  IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14415-1-PBS bindet in WB, IHC, IP, Indirect ELISA UBE2L3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2L3 fusion protein Ag5758
• Vollständiger Name: ubiquitin-conjugating enzyme E2L 3
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC053368
• Gene symbol: UBE2L3
• Gene ID (NCBI): 7332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).