UBE2L6 Polyklonaler Antikörper

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• KD/KO Validated

UBE2L6 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17278-1-PBS

Synonyme

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  WB analysis of Jurkat using 17278-1-AP (same clone as 17278-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17278-1-AP (UBE2L6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 17278-1-AP (same clone as 17278-1-PBS)

  WB result of UBE2L6 antibody (17278-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE2L6 transfected Jurkat cells. This data was developed using the same antibody clone with 17278-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 17278-1-AP (same clone as 17278-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17278-1-AP (UBE2L6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17278-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 17278-1-AP (same clone as 17278-1-PBS)

  IP result of anti-UBE2L6 (IP:17278-1-AP, 4ug; Detection:17278-1-AP 1:1000) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 17278-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17278-1-AP (same clone as 17278-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBE2L6 antibody (17278-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17278-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17278-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA UBE2L6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2L6 fusion protein Ag11045
• Vollständiger Name: ubiquitin-conjugating enzyme E2L 6
• Berechnetes Molekulargewicht: 153 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC032491
• Gene symbol: UBE2L6
• Gene ID (NCBI): 9246
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.