UBC13 Polyklonaler Antikörper

UBC13 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 10243-1-AP

Synonyme

BLU, UBC13, UbcH ben, UBE2N, Ubiquitin carrier protein N, Ubiquitin protein ligase N

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Galerie der Validierungsdaten

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  WB analysis of mouse thymus using 10243-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10243-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 10243-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10243-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10243-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10243-1-AP

  IP Result of anti-UBC13 (IP:10243-1-AP, 4ug; Detection:10243-1-AP 1:600) with MCF-7 cells lysate 1280ug.

• 

  IHC staining of human breast cancer using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, humanes Herzgewebe, Jurkat-Zellen, Maus-Dünndarmgewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

10243-1-AP bindet in WB, IP, IHC, ELISA UBC13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBC13 fusion protein Ag0292
• Vollständiger Name: ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC000396
• Gene symbol: UBE2N
• Gene ID (NCBI): 7334
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.; Authors - Floriana Mulas
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
mouse	WB,IP	Nat Commun; FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke; Authors - Bin Li
human	WB,IHC	Mol Biomed; SP1 transcriptionally regulates UBE2N expression to promote lung adenocarcinoma progression; Authors - Jianjun Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jessica (Verified Customer) (11-14-2019)

Antibody works well for detecting the endogenous Ubc13 protein in 293T cells. Strong signal and few non specific bands.

• Applications: Western Blot,
• Cell Tissue Type: 293T cells