UBE2O Polyklonaler Antikörper

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• KD/KO Validated

UBE2O Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15812-1-PBS

Synonyme

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• 

  WB analysis of HeLa using 15812-1-AP (same clone as 15812-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15812-1-AP (UBE2O antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 15812-1-AP (same clone as 15812-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 15812-1-AP (UBE2O antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15812-1-AP (same clone as 15812-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15812-1-AP (UBE2O antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 15812-1-AP (same clone as 15812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15812-1-AP (UBE2O Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 15812-1-AP (same clone as 15812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15812-1-AP (UBE2O Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15812-1-PBS bindet in WB, IHC, Indirect ELISA UBE2O und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2O fusion protein Ag8580
• Vollständiger Name: ubiquitin-conjugating enzyme E2O
• Berechnetes Molekulargewicht: 1292 aa, 141 kDa
• Beobachtetes Molekulargewicht: 150 kDa, 200-230 kDa
• GenBank-Zugangsnummer: BC007822
• Gene symbol: UBE2O
• Gene ID (NCBI): 63893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2O, also named as KIAA1734, belongs to the ubiquitin-conjugating enzyme family. It catalyzes the covalent attachment of ubiquitin to other proteins. UBE2O has a calculated molecular mass of 141 kDa, and many bands can be detected as 140-170 kDa and 200-230 kDa (PMID: 28774900, 23455153, 28774922, 11311559).