UBE2S Polyklonaler Antikörper

UBE2S Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18219-1-AP

Synonyme

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 18219-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18219-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 18219-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18219-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 18219-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, COLO 320-Zellen, Maus-Eierstockgewebe, MCF-7-Zellen, MDA-MB-453s-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18219-1-AP bindet in WB,ELISA UBE2S und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2S fusion protein Ag12978
• Vollständiger Name: ubiquitin-conjugating enzyme E2S
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC004236
• Gene symbol: UBE2S
• Gene ID (NCBI): 27338
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).