UBE3A Monoklonaler Antikörper

UBE3A Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

3D6H9

Kat-Nr. : 60038-1-PBS

Synonyme

3D6H9, ANCR, AS, E6 AP, E6AP

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  WB analysis of HeLa using 60038-1-Ig (same clone as 60038-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60038-1-Ig (UBE3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP51408-1

  Sample test of MP51408-1, UBE3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60038-1-PBS. Detection antibody: 60977-1-PBS.

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  IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51408-1

  Cytometric bead array standard curve of MP51408-1, UBE3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60038-1-PBS. Detection antibody: 60977-1-PBS. Standard:Ag17546. Range: 3.125-400 ng/mL .

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Geprüfte Anwendungen

Produktinformation

60038-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA, Sample test UBE3A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UBE3A fusion protein Ag0346
• Vollständiger Name: ubiquitin protein ligase E3A
• Berechnetes Molekulargewicht: 852 aa, 98 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC002582
• Gene symbol: UBE3A
• Gene ID (NCBI): 7337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479 ). It has 3 isoforms produced by alternative splicing.