UBE4B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBE4B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 18148-1-AP

Synonyme

EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2

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  WB analysis using 18148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18148-1-AP (UBE4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18148-1-AP

  WB result of UBE4B antibody (18148-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE4B transfected HeLa cells.

• 

  WB analysis of mouse testis using 18148-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18148-1-AP (UBE4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18148-1-AP

  IP result of anti-UBE4B (IP:18148-1-AP, 4ug; Detection:18148-1-AP 1:1000) with HeLa cells lysate 2320 ug.

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  IHC staining of human stomach cancer using 18148-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18148-1-AP (UBE4B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18148-1-AP

  Immunofluorescent analysis of HeLa cells using 18148-1-AP (UBE4B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, K-562-Zellen, Maushodengewebe, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Produktinformation

18148-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA UBE4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE4B fusion protein Ag12836
• Vollständiger Name: ubiquitination factor E4B (UFD2 homolog, yeast)
• Berechnetes Molekulargewicht: 1173 aa, 133 kDa
• Beobachtetes Molekulargewicht: 146 kDa
• GenBank-Zugangsnummer: BC093696
• Gene symbol: UBE4B
• Gene ID (NCBI): 10277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.

Publikationen

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu
human	WB	Mol Carcinog; Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma.; Authors - Xiao-Fei Zhang
human	WB	Proteomics; SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells.; Authors - Hongyu Zhang
human	WB,IHC	Onco Targets Ther; The Roles of Ubiquitination Factor E4B (UBE4B) in the Postoperative Prognosis of Patients with Renal Cell Carcinoma and in Renal Tumor Cells Growth and Metastasis.; Authors - Xu-Qiong Huang ; KD Validated
human	IHC	Pathol Res Pract; UBE4B promotes the development of lung adenocarcinoma by enhancing proliferation, migration and glycolysis via PP2A/AKT signaling.; Authors - Song Wu
human	WB	Biochim Biophys Acta Gene Regul Mech; UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated NRF1 cleavage; Authors - Xianyan Hu ; KD Validated