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UBE4B Rekombinanter Antikörper

UBE4B Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242218E4

Kat-Nr. : 85332-2-PBS

Synonyme

EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2

Filter:
  • Western Blot (WB) analysis of various lysates using UBE4B Recombinant antibody (85332-2-RR)

    WB analysis using 85332-2-RR (same clone as 85332-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85332-2-RR (UBE4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using UBE4B Recombinant antibody (85332-2-RR)

    WB analysis of HeLa using 85332-2-RR (same clone as 85332-2-PBS)

    WB result of UBE4B antibody (85332-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE4B transfected HeLa cells. This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBE4B Recombinant antibody (85332-2-RR)

    IF Staining of HeLa using 85332-2-RR (same clone as 85332-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE4B antibody (85332-2-RR, Clone: 242218E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85332-2-RR

    Affinity and Kinetic Characterization of 85332-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85332-2-RR against Human UBE4B were performed. The affinity constant is 0.895 nM.

Geprüfte Anwendungen

Produktinformation

85332-2-PBS bindet in WB, IF/ICC, Indirect ELISA UBE4B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen UBE4B fusion protein Ag12836
Vollständiger Name ubiquitination factor E4B (UFD2 homolog, yeast)
Berechnetes Molekulargewicht 1173 aa, 133 kDa
Beobachtetes Molekulargewicht140-146 kDa
GenBank-ZugangsnummerBC093696
Gene symbol UBE4B
Gene ID (NCBI) 10277
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.