UBE4B Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

UBE4B Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242218E4

Kat-Nr. : 85332-2-RR

Synonyme

EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2

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  WB analysis using 85332-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85332-2-RR (UBE4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 85332-2-RR

  WB result of UBE4B antibody (85332-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE4B transfected HeLa cells.

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  IF Staining of HeLa using 85332-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE4B antibody (85332-2-RR, Clone: 242218E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85332-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85332-2-RR against Human UBE4B were performed. The affinity constant is 0.895 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, K-562-Zellen, Raji-Zellen, Rattenhodengewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85332-2-RR bindet in WB, IF/ICC, ELISA UBE4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UBE4B fusion protein Ag12836
• Vollständiger Name: ubiquitination factor E4B (UFD2 homolog, yeast)
• Berechnetes Molekulargewicht: 1173 aa, 133 kDa
• Beobachtetes Molekulargewicht: 140-146 kDa
• GenBank-Zugangsnummer: BC093696
• Gene symbol: UBE4B
• Gene ID (NCBI): 10277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.