UBF1 Polyklonaler Antikörper

UBF1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 20660-1-AP

Synonyme

Autoantigen NOR 90, NOR 90, UBF, UBF 1, UBF1, UBTF, Upstream binding factor 1

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Galerie der Validierungsdaten

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  WB analysis of HT-1080 using 20660-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20660-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20660-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20660-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 20660-1-AP (UBF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20660-1-AP bindet in WB, IF, ELISA UBF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: upstream binding transcription factor, RNA polymerase I
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: NM_014233
• Gene symbol: UBTF
• Gene ID (NCBI): 7343
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Detectable but weak signal in the nucleoli

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

UBF1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:20660-1-AP)