UBQLNL Polyklonaler Antikörper

UBQLNL Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16400-1-AP

Synonyme

Ubiquilin-like protein, Ubiquilin like protein, ubiquilin like

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  WB analysis of human brain using 16400-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16400-1-AP (UBQLNL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16400-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16400-1-AP (UBQLNL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16400-1-AP

  IP result of anti-UBQLNL (IP:16400-1-AP, 4ug; Detection:16400-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human colon using 16400-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16400-1-AP (UBQLNL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16400-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16400-1-AP (UBQLNL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16400-1-AP

  Immunofluorescent analysis of A431 cells using 16400-1-AP (UBQLNL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of A431 using 16400-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBQLNL antibody (16400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

16400-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA UBQLNL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBQLNL fusion protein Ag9496
• Vollständiger Name: ubiquilin-like
• Berechnetes Molekulargewicht: 475 aa, 53 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC012183
• Gene symbol: UBQLNL
• Gene ID (NCBI): 143630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBQLNL also named as Ubiquilin like protein, encodes a 475 amino acid protein. The specific function of it is still non-known. UBQLNL may have an association with type 2 diabetes, but the mechanism is supposed be studied in the further research. The subcellular localization of UBQLNL is nucleus. The expression of it is detected in some tissues, such as brain ,testis, heart and liver.

Publikationen

Species	Application	Title
mouse	WB,IF	Mol Med Rep; Decreased expression of ubiquilin‑1 following neonatal hypoxia‑ischemic brain injury in mice.; Authors - Li Luo