UBR4 Polyklonaler Antikörper

UBR4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15966-1-PBS

Synonyme

---

• 

  WB analysis using 15966-1-AP (same clone as 15966-1-PBS)

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 15966-1-AP (UBR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

  IP result of anti-UBR4 (IP:15966-1-AP, 4ug; Detection:15966-1-AP 1:2000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15966-1-AP (same clone as 15966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody (15966-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

15966-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA UBR4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBR4 fusion protein Ag8740
• Vollständiger Name: ubiquitin protein ligase E3 component n-recognin 4
• Berechnetes Molekulargewicht: 5183 aa, 570 kDa
• Beobachtetes Molekulargewicht: 570 kDa, 270 kDa
• GenBank-Zugangsnummer: BC018694
• Gene symbol: UBR4
• Gene ID (NCBI): 23352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4), also named P600, is an E3 ligase that can bind N-end rule substrates. UBR4-deficient mice die during embryogenesis and exhibit pleiotropic abnormalities (PMID: 23431188). UBR4 plays a role in membrane morphogenesis and extracellular matrix-mediated cell survival and signaling, human papillomavirus (HPV) E7-mediated cellular immortalization, and migration and development of neurons (PMID: 33658508, 25084275). The full-length UBR4 protein has a predicted size of ~570 kDa.