UBR5 Polyklonaler Antikörper

UBR5 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 22782-1-AP

Synonyme

EDD1, EDD, EC:2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, E3 ubiquitin-protein ligase UBR5

---

• 

  IHC staining of mouse testis using 22782-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22782-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22782-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22782-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 22782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 22782-1-AP (UBR5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

22782-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA UBR5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBR5 fusion protein Ag18753
• Vollständiger Name: ubiquitin protein ligase E3 component n-recognin 5
• Berechnetes Molekulargewicht: 2799 aa, 309 kDa
• Beobachtetes Molekulargewicht: 309 kDa
• GenBank-Zugangsnummer: BC137234
• Gene symbol: UBR5
• Gene ID (NCBI): 51366
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	IF	Nat Commun; UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors; Authors - Duomeng Yang
human	IF	J Gastroenterol Hepatol; C2orf40 inhibits hepatocellular carcinoma through interaction with UBR5.; Authors - Yue Wu
human	WB,IF	J Virol; UBR5 Acts as an Antiviral Host Factor against MERS-CoV via Promoting Ubiquitination and Degradation of ORF4b; Authors - Yuzheng Zhou
human,mouse	WB,IHC,IF,CoIP	Sci China Life Sci; SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1; Authors - Hao Wang
human	IF	J Transl Med; Inhibition of the E3 ligase UBR5 stabilizes TERT and protects vascular organoids from oxidative stress; Authors - Haijing Zhao