UBR5 Monoklonaler Antikörper

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UBR5 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E7A6

Kat-Nr. : 66937-1-PBS

Synonyme

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

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  WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

  WB result of UBR5 antibody (66937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBR5 transfected HeLa cells. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66937-1-Ig (same clone as 66937-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66937-1-Ig (same clone as 66937-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66937-1-Ig (same clone as 66937-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66937-1-Ig (same clone as 66937-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66937-1-PBS bindet in WB, IHC, Indirect ELISA UBR5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UBR5 fusion protein Ag18753
• Vollständiger Name: ubiquitin protein ligase E3 component n-recognin 5
• Berechnetes Molekulargewicht: 2799 aa, 309 kDa
• Beobachtetes Molekulargewicht: 309 kDa
• GenBank-Zugangsnummer: BC137234
• Gene symbol: UBR5
• Gene ID (NCBI): 51366
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.