UBR7 Polyklonaler Antikörper

UBR7 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16903-1-AP

Synonyme

C14orf130, EC:2.3.2.27, N recognin 7, N-recognin-7, Putative E3 ubiquitin-protein ligase UBR7

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  WB analysis using 16903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16903-1-AP (UBR7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16903-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBR7 antibody (16903-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16903-1-AP bindet in WB, IF/ICC, ELISA UBR7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBR7 fusion protein Ag10445
• Vollständiger Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-60 kDa
• GenBank-Zugangsnummer: BC015046
• Gene symbol: UBR7
• Gene ID (NCBI): 55148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBR7 (C14orf130), is a 425-amino-acid putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. The N-end rule pathway is a ubiquitin-dependent system where E3 ligases called N-recognins, including UBR1, UBR2 and UBR7 (N-recognin-7), leading to target protein ubiquitination and subsequent degradation (PMID: 19008229). UBR7 contains a PHD-type zinc finger and a UBR-type zinc finger domain (PMID: 14702039), and probably undergoes acetylation (PMID: 19413330). Till date its precise function is not well known. Proteintech's UBR7 antibody 16903-1-AP is able to detect UBR7 with a theoretical MW of 48 kDa.