UCHL1/PGP9.5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

UCHL1/PGP9.5 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1C9E11

Kat-Nr. : 66230-1-Ig

Synonyme

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCH-L1, UCHL1/PGP9.5

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Galerie der Validierungsdaten

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  WB analysis using 66230-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of Y79 using 66230-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66230-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 66230-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UCHL1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of Y79 using 66230-1-Ig

  1X10^6 Y79 cells were intracellularly stained with 0.2 ug Anti-Human UCHL1/PGP9.5 (66230-1-Ig, Clone:1C9E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Hausschwein-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Maus-Cerebellum-Gewebe, Maushirngewebe, PC-12-Zellen, Ratten-Cerebellum-Gewebe, Rattenhirngewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

66230-1-Ig bindet in WB, IHC, IF, FC, ELISA UCHL1/PGP9.5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UCHL1/PGP9.5 fusion protein Ag6547
• Vollständiger Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC000332
• Gene symbol: UCHL1
• Gene ID (NCBI): 7345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)

Publikationen

Species	Application	Title
human	WB	Theranostics; UCH-L1-mediated Down-regulation of Estrogen Receptor α Contributes to Insensitivity to Endocrine Therapy for Breast Cancer.; Authors - Xi-Sha Chen ; KD Validated
human	IF	Dig Dis Sci; P2Y1, P2Y2, and TRPV1 Receptors Are Increased in Diarrhea-Predominant Irritable Bowel Syndrome and P2Y2 Correlates with Abdominal Pain.; Authors - Yumei Luo
pig	WB, IF	Reprod Domest Anim; Isolation and in vitro expansion of porcine spermatogonial stem cells.; Authors - Xin Zhao
rat	WB	ORL J Otorhinolaryngol Relat Spec; Role of the Ubiquitin C-Terminal Hydrolase L1-Modulated Ubiquitin Proteasome System in Auditory Cortex Senescence.; Authors - Ya Zhang
mouse	IHC	Discov Oncol; Oxaliplatin-induced peripheral neuropathy can be minimized by pressurized regional intravascular delivery in an orthotopic murine pancreatic cancer model.; Authors - Jayanth Surya Narayanan Shankara Narayanan
mouse	IF	PLoS Biol; The RSK2-RPS6 axis promotes axonal regeneration in the peripheral and central nervous systems; Authors - Charlotte Decourt

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