UCH-L1/PGP9.5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

UCH-L1/PGP9.5 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(18)

CloneNo.

1C9E11

Kat-Nr. : 66230-1-Ig

Synonyme

PGP9.5, UCHL1, UCHL1/PGP9.5, 1C9E11, EC:3.4.19.12

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  WB analysis using 66230-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of Y79 using 66230-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66230-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 66230-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 66230-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UCH-L1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 66230-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using UCH-L1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of A549 using 66230-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using UCH-L1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of Y79 using 66230-1-Ig

  1x10^6 Y79 cells were intracellularly stained with 0.4 ug UCHL1/PGP9.5 Monoclonal antibody (66230-1-Ig, Clone:1C9E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, PC-12-Zellen, Hausschwein-Hirngewebe, Hausschwein-Cerebellum-Gewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 10 publications below

Produktinformation

66230-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA UCH-L1/PGP9.5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UCH-L1/PGP9.5 fusion protein Ag6547
• Vollständiger Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC000332
• Gene symbol: UCHL1
• Gene ID (NCBI): 7345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)

Publikationen

Species	Application	Title
human	WB	Theranostics; UCH-L1-mediated Down-regulation of Estrogen Receptor α Contributes to Insensitivity to Endocrine Therapy for Breast Cancer.; Authors - Xi-Sha Chen ; KD Validated
human	IHC	Sci Rep; Gingival proteomics reveals the role of TGF beta and YAP/TAZ signaling in Raine syndrome fibrosis; Authors - Cláudio Rodrigues Rezende Costa
mouse	IF	Am J Physiol Gastrointest Liver Physiol; Carbon monoxide produced by HO-1 upregulation is the main factor behind the abnormal motility seen in experimental ulcerative colitis in mice; Authors - Mengchao Zhao
rat	IHC	World J Gastroenterol; Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1/nuclear factor-erythroid-2-related factor 2/heme oxygenase-1 signaling pathway; Authors - Kai-Di Chen
mouse	IF	Toxicol Appl Pharmacol; Ursolic acid alleviates paclitaxel-induced peripheral neuropathy through PPARγ activation; Authors - Yulian Yang
pig	IF	Animals (Basel); Single-Cell RNA Sequencing Reveals an Atlas of Meihua Pig Testis Cells; Authors - Mao Zhang