UCH-L1/PGP9.5 Rekombinanter Antikörper

UCH-L1/PGP9.5 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241107B2

Kat-Nr. : 84154-4-RR

Synonyme

PGP9.5, UCHL1, UCHL1/PGP9.5, 241107B2, EC:3.4.19.12

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  WB analysis using 84154-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84154-4-RR (UCHL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84154-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-4-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

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  IF Staining of mouse brain using 84154-4-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Neuro-2a using 84154-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84154-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84154-4-RR against Human UCHL1/PGP9.5 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:125-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84154-4-RR bindet in WB, IHC, IF/ICC, IF-P, ELISA UCH-L1/PGP9.5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UCH-L1/PGP9.5 fusion protein Ag6490
• Vollständiger Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC000332
• Gene symbol: UCHL1
• Gene ID (NCBI): 7345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).