UCH-L1/PGP9.5 Rekombinanter Antikörper

UCH-L1/PGP9.5 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241107B3

Kat-Nr. : 84154-5-PBS

Synonyme

PGP9.5, UCHL1, UCHL1/PGP9.5, 241107B3, EC:3.4.19.12

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  WB analysis using 84154-5-RR (same clone as 84154-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84154-5-RR (UCHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84154-5-RR (same clone as 84154-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-5-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84154-5-RR (same clone as 84154-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-5-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84154-5-RR (same clone as 84154-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-5-RR, Clone: 241107B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84154-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84154-5-RR against Human UCH-L1/PGP9.5 were performed. The affinity constant is 6.10 nM.

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Geprüfte Anwendungen

Produktinformation

84154-5-PBS bindet in WB, IHC, IF-P, Indirect ELISA UCH-L1/PGP9.5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UCH-L1/PGP9.5 fusion protein Ag6490
• Vollständiger Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC000332
• Gene symbol: UCHL1
• Gene ID (NCBI): 7345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).