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UCHL1/PGP9.5 Monoklonaler Antikörper

UCHL1/PGP9.5 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1C9E11

Kat-Nr. : CL594-66230

Synonyme

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCHL1/PGP9.5

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of Y79 cells using CoraLite®594-conjugated UCHL1/PGP9.5 Monoclonal an (CL594-66230)

    FC experiment of Y79 using CL594-66230

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human UCHL1/PGP9.5 (CL594-66230, Clone:1C9E11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCY79-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66230 bindet in FC (Intra) UCHL1/PGP9.5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen UCHL1/PGP9.5 fusion protein Ag6547
Vollständiger Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 27 kDa
GenBank-ZugangsnummerBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Protokolle

Produktspezifische Protokolle
FC protocol for CL594 UCHL1/PGP9.5 antibody CL594-66230Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouse,ratFC (Intra)

Br J Pharmacol

Dopamine regulates colonic glial cell-derived neurotrophic factor secretion through cholinergic dependent and independent pathways

Authors - Xiao-Li Zhang
View Article