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UCHL3 Rekombinanter Antikörper

UCHL3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242015A10

Kat-Nr. : 84542-6-PBS

Synonyme

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

Filter:
  • Western Blot (WB) analysis of various lysates using UCHL3 Recombinant antibody (84542-6-RR)

    WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UCHL3 Recombinant antibody (84542-6-RR)

    WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UCHL3 Recombinant antibody (84542-6-RR)

    IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84542-6-RR

    Affinity and Kinetic Characterization of 84542-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84542-6-RR against Human UCHL3 were performed. The affinity constant is 2.45 nM.

Geprüfte Anwendungen

Produktinformation

84542-6-PBS bindet in WB, IHC, Indirect ELISA UCHL3 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen UCHL3 fusion protein Ag3053
Vollständiger Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Berechnetes Molekulargewicht 26 kDa
Beobachtetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerBC018125
Gene symbol UCHL3
Gene ID (NCBI) 7347
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.